2007 - Roczniki Akademii Rolniczej w Poznaniu 383, Seria Ogrodnictwo 41: 153-158

Bożena Pawłowska, Bożena Szewczyk-Taranek

Aktywność fotosyntetyczna przylaszczki pospolitej (Hepatica nobilis Schreb.)
rozmnażanej w kulturach in vitro

Z Katedry Roślin Ozdobnych
Akademii Rolniczej im. Hugona Kołłątaja w Krakowie

Abstract. Adaptation rate of hepatica propagated by in vitro organogenesis and rooting was 65%. Studies with the use of Mini PAM fluorometer showed low photosynthetic activity of hepatica leaves in in vitro cultures, but it increased after several weeks of acclimatization in newly developed leaves.

Key words: Hepatica nobilis, in vitro, chlorophyll fluorescence, mini PAM 2000

 

Photosynthetic activity of hepatica (Hepatica nobilis schreb.) propagated in vitro

Summary

Hepatica micropropagation in vitro on MS medium supplemented with 5 μM BA and 0,5 μM NAA allowed to obtain high propagation rate of lateral buds (6.5 buds/explant per 3-week cycle). Plants were chilled before rooting at 4°C and placed on MS medium containing 10 μM NAA. Plants established after 15-week acclimatization in a greenhouse (65%) were planted in pots; 75% of plants survived. Measurement of chlorophyll fluorescence in hepatica leaves using Mini PAM fluorometer (Walz, Germany) demonstrated low photosynthetic activity of hepatica cultured in vitro, but this activity rose in successive weeks of acclimatization.